Three Novel Ascomycetous Yeast Species Isolated from Plant Leaves and Wild Mushrooms Collected in Thailand

Abstract: Four yeast strains representing three novel species were isolated from two sources in Thailand, one from the leaf surface of a tall-stilt mangrove (Rhizophora apiculata, strain DMKU-RG45) and three from the fruiting bodies of wild mushrooms (strains DMKU-TM03, DMKU-SM11, and DMKU-SM28) collected in Thailand. Sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region revealed that these four strains differed by 0.87–8.68% and 2.07–13.55% in their nucleotide divergence from their closest described species in the sequences of the D1/D2 domain of the LSU rRNA gene and the ITS region, respectively. In phylogenetic analyses, the strain DMKU-RG45 belonged to the genus Cyberlindnera and was distinct from other recognized species in this genus, whereas the strains DMKU-TM03, DMKU-SM11, and DMKU-SM28 were members of the genus Blastobotrys. However, they were in two different positions, each of which was distinct from closely related species. Based on molecular analyses and phenotypic characteristics, the strain DMKU-RG45 is proposed as Cyberlindnera sirindhorniae f.a., sp. nov. (the holotype is TBRC 19863T; MycoBank No. is MB 858763). The other three strains (DMKU-TM03, DMKU-SM11, and DMKU-SM28) are proposed as two novel Blasto-botrys species. Blastobotrys princeps f.a., sp. nov. is proposed to accommodate the strain DMKU-TM03 (the holotype is TBRC 19382T; MycoBank No. is MB 858764). Blastobotrys siamensis f.a., sp. nov. is proposed to accommodate both strains DMKU-SM11 and DMKU-SM28 (the holotype is TBRC 19380T; MycoBank No. is MB 858765).

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เอกสารอ้างอิง: Khunnamwong, P., P. Gungprakhon, P. Nualthaisong, B. Sakolrak, N. Srisuk and S. Limtong. 2025. Three Novel Ascomycetous Yeast Species Isolated from Plant Leaves and Wild Mushrooms Collected in Thailand. Tropical Natural History. (8): 399-414.